Selected abstracts on phorbol ester action in cell culture

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U.S. Dept. of Health and Human Services, Public Health Service, National Institutes of Health, National Techinal Information Service [distributor] , Bethesda, Md, Springfield, Va
Cocarcinogens -- Abstracts., Esters -- Abstr
Other titlesPhorbol ester action in cell culture.
StatementThomas G. O"Brien, consulting reviewer.
SeriesOncology overview
ContributionsO"Brien, Thomas G., International Cancer Research Data Bank.
The Physical Object
Paginationix, 140 p. ;
ID Numbers
Open LibraryOL17550487M

Get this from a library. Selected abstracts on phorbol ester action in cell culture. [Thomas G O'Brien; International Cancer Research Data Bank.; Cancer Information Dissemination and Analysis Center (CIDAC) for Carcinogenesis Information.;].

Author(s): O'Brien,Thomas G; International Cancer Research Data Bank.; Cancer Information Dissemination and Analysis Center (CIDAC) for Carcinogenesis Information.

Title(s): Selected abstracts on phorbol ester action in cell culture/ Thomas G. O'Brien, consulting reviewer. The study reported bio-assay assisted isolation of phorbol ester in optimized solid and liquid system tissue culture. The effect of the compound on human leukemic cell line (Jurkat J6) was conformed using MTT and SRB assays.

The extract was analyzed using HPTLC in standard phorbol myristate acetate equivalent. Phorbol ester composition in seed oil was found to be ±%.Cited by: 6. On the other hand, the ultraviolet irradiation sensitivity of phorbol ester induction in normal chicken embryo fibroblasts was the same as in transformed cells, indicating that the synergism of transformation and phorbol esters is probably not due to different pathways of PA induction.}, doi = {/MCB}, journal = {Mol.

Cell. Biol. The relationship between 5-hydroxyeicosatetraenoic acid (5-HETE) and calcium-activated, phospholipid-dependent protein kinase (protein kinase C) in prolactin (PRL) release was investigated in rat anterior pituitary cells.

Arachidonic acid or 5-HETE, a 5-lipoxygenase metabolite of arachidonic acid, is known to cause a significant concn.-dependent increase in PRL by: 1. Abstracts on natural anti-inflammatories and evaluation of their ability to inhibit phorbol ester-induced prostaglandin E2 production.

production in the cell culture system. These findings suggest that the mechanism of action of taiwanin C in the inhibition of PGE(2) production is the direct inhibition of COX enzymatic activity.". Selectivity of curcumin and its analogs for conventional PKCs, 39th MALTO abstract book,p J.

Das, S. Pany, S. Xu, V. Shah and G. Roman. Presynaptic Munc is a potential target of alcohol action, in From Innovation in Nucleic acid research to regulation of. Cell Culture.

Description Selected abstracts on phorbol ester action in cell culture FB2

LLC-PK 1 cells, differentiated renal epithelial cells with marker properties of the renal proximal tubule, were a gift of Dr. Robert Hull (Eli Lilly & Co., Indianapolis, IN) (Hull et al., ; Mullin et al., ) and were used between passages and The procedures for culture of the LLC-PK 1 cell line have been described previously (Mullin and O'Brien, ; Mullin et al.

- 9-acridone had LC50 and LC90 values of and µg/ml, respectively. Secofuroquinoline alkaloids. Selected abstracts on phorbol ester action in cell culture book Molecular Biology of the Cell Vol.

10, No. 5 Articles Free Access Human munc13 Is a Diacylglycerol Receptor that Induces Apoptosis and May Contribute to Renal Cell Injury in Hyperglycemia Yong Song.

Most phorbol ester PKC-activators such as phorbol myristate acetate are potent mitogens [,]. However, ingenol 3-mebutate displays anti-tumor [ ] and anti-leukemic effects [ ] while also promoting PKC.

Thus, phorbol ester can either stimulate or inhibit cell proliferation, depending on cell type and signal-transduction pathway (e.g., see Huang et al.

The choice of an appropriate surrogate is an absolutely crucial consideration to extrapolate from in vitro to in vivo response.

26 Via DP, Pons L, Dennison DK, Fanslow AE, Bernini F. Induction of acetyl-LDL receptor activity by phorbol ester in human monocyte cell line THP J Lipid Res. In conclusion, the use of mM Calcium and 10μM Y in HEKn monocultures provides the opportunity to culture primary human keratinocytes without a cell feeder layer for extended periods of.

The action of an alkyl-lysophospholipid (ALP), ETCH3, on clonogenicity, 3H-TdR uptake, and cell numbers was tested in two human leukemic cell lines, HL60 and K, and short-term human leukemic. The morpholinoethyl ester of MPA (MMF, Fig. 4) was found to have improved bioavailability in primates as compared with MPA (Lee et al., ).

The ester is rapidly hydrolysed to yield MPA, both in human peripheral blood mononuclear cell cultures and in vivo. MMF proved suitable for pharmaceutical formulation and was selected for development. Regulation and function of cyclooxygenase-2 (COX-2) in bone. COX-2 is responsible for most prostaglandin regulation in bone.

Ongoing research projects include examination of transcriptional regulation of COX-2 in osteoblastic cells and examination of the effects of COX-2 gene disruption on bone resorption and formation.

A comparative study of releasing and nonreleasing human basophils: Nonreleasing basophils lack an early component of the signal transduction pathway that follows IgE cross-linking Kirn-Lien Nguyen, MD, Steven Gillis, PhD,* and Donald W.

MacGlashan, Jr., MD, PhD Baltimore, Md., and Seattle, Wash. Basophils from approximately one fifth of the population were found to be unresponsive. Both HDL 3 and LDL activate signal transduction cascades in several cell types.

The activation of PI-PLC in response to HDL 3 and LDL occurs in vascular smooth muscle cells and type II pneumocytes. 1 2 PI-PLC–catalyzed hydrolysis of PIP 2 yields two second messengers, InsP 3 and 1,2-DAG. In accordance with the known second-messenger functions of InsP 3 3 and 1,2-DAG, 4 both lipoproteins.

Insulin maintains vascular smooth muscle cell (VSMC) quiescence yet can also promote VSMC migration. The mechanisms by which insulin exerts these contrasting effects were examined using α-smooth muscle actin (α-SMA) as a marker of VSMC phenotype because α-SMA is highly expressed in quiescent but not migratory VSMC.

Insulin alone maintained VSMC quiescence and modestly. Sieber-Blum M and Sieber F: Tumor-promoting phorbol ester affects neural crest cell development.

Europ J Cell BiolEurop J Cell Biol Specialized plant terpenoids have found fortuitous uses in medicine due to their evolutionary and biochemical selection for biological activity in animals.

However, these highly functionalized natural products are produced through complex biosynthetic pathways for which we have a complete understanding in only a few cases. Here we review some of the most effective and promising plant.

Two spirosteroid analogues were synthesized and evaluated for their in vitro neuroprotective activities in PC12 cells, against glutamate-induced excitotoxicity and mitochondrial damage in glucose deprivation conditions, as well as their anti-inflammatory potential in LPS/IFNγ-stimulated microglia primary cultures.

We also evaluated the in vitro anti-excitotoxic and anti-inflammatory. This suggests that the antidiabetic action of these compounds may involve PPARy-mediated transcription of adipose genes involved in lipid and carbohydrate metabolism.

To further understand the action of TZDs, we explored the effects of these agents on differentiation of C3H1OTI/2 cells, a pluripotent stem cell-line of mesodermal origin. Book of Abstracts. Bremen, Germany action in immune cell recruitment must be clarified.

We investigated whether serotonin alters the phorbol ester PDBu induced S upregulation but not Y-phosphorylation of Syk in intact platelets as well in platelet lysates, which. In the last 15 years the hypothetical concept of cocarcinogenesis has been substantiated by chemical, biochemical, and biologic investigation of the polyfunctional, diterpene mono- and diester type promoters (and stimulators) of initiation, originating from plant species.

Thus, the long suspected concept of amplification of carcinogenesis by non-carcinogens was finally established. Cell Phone (& Smartphone) Radiation Poses a Serious Biologic Health Risk "Cherry () predicted that since electrical workers and physiotherapists are occupationally exposed to electromagnetic fields that are about a million times higher than the Schumann Resonance signal, they would have higher rates of cancer, cardiac reproductive and neurological effects.

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Full text of "Proceedings of the Society for Experimental Biology and Medicine". 0 CONTENTS OF OTHER CANCER JOURNALS 0 CANCER RESEARCH-VOL.

46,NO. 12, DECEMBER Editorial Office: Fels Research Institute, Temple University School of Medicine, Phdadelphia, PAUSA Chromosome translocations and human cancer, by Carlo M. Croce.

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DNA damage in cultured L cells by 2-haloethyl esters of (methylsulfony1)methanesulfonic acid, by 10 Ann Alexander. General Overview: Dr. Marja Hurley is Professor of Medicine and Orthopedics and Member of the Institute of Systems Genomics at the University of Connecticut School of is the first woman of color to receive the doctor of medicine from UConn School of Medicine and the first to be a tenured full professor at the UConn Health School of Medicine.

Birds fed diets 2 and 3 had superior (p ) haemoglobin content, packed cell volume and red blood cell values. Total cholesterol decreased significantly (p ) in the bitter leaf and ginger diets with values decreasing from mg per ml in control diet to mg per ml (36 % decrease) in diet 3.The faculty in the Department of Pharmaceutical and Biomedical Sciences have diverse expertise in pharmacology, toxicology, medicinal chemistry, pharmaceutics, physiology, cellular and molecular biology, biotechnology, immunology, and microbiology.

Primary areas of research include inflammation, targeted drug delivery, cancer biology, diabetes, cardiovascular diseases and team based learning.EPA/ TR INVESTIGATION OF SELECTED POTENTIAL ENVIRONMENTAL CONTAMINANTS: EPOXIDES Dennis A.

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Bogyo Sheldon S. Lande William M. Meylan Philip H. Howard Joseph Santodonato March FINAL REPORT Contract No. SRC No. L Project Officer - Frank J. Letkiewicz Prepared for: Office of Toxic Substances U.S.

Environmental Protection .